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Human Immunodeficiency Virus Viral Load

Alias: HIV, HIV RNA, HIV-1

Discipline: Virology

Test information: This assay is used for the detection and quantification of HIV-1 RNA in human plasma from HIV-1 infected individuals, to monitor response to treatment. This assay is NOT intended to be used as a blood donor screening test for HIV-1 or as a diagnostic test to confirm the presence of HIV-1 infection.

Availability: Available locally Monday - Friday locally during routine hours: 08:45 - 17:15.

Turnaround Time: Same Day

Related Tests:

Specimen Type(s)
  • EDTA Whole Blood;
Other Acceptable Specimen Type(s)
Specimen Container - Adult
  • Gold Top SST BD;
Specimen Container - Paediatric
  • N/A;
  • HIV-1 RNA ;
Part of a test profile?
Volume (min) of sample to be sent to laboratory
Whole Blood: 10 - 20mL
Please note this test requires a minimum of 1 mL of plasma to perform.
Patient Preparation, Sample Handling and Transport
  • Specimens should be transported to the laboratory as soon as possible
  • Whole blood samples are stable up to 24 hours at 2 - 30 °C
Maximum add on time
Units and Reference Ranges / Interpretation
Units: copies/mL
Linear Range: 40 to 10,000,000 I copies/mL
Limit of Quantification: 40 copies/mL
Limit of Detection: Please contact laboratory, this varies between HIV-1 subtypes and groups but is <22.3 copies/mL for all sub types and groups detected by this assay.
UKAS number
UKAS accredited test?
Patients who have received CAR-T therapies may display positive results as a result of the presence of the LTR target within certain chimeric antigen receptor T-cell (CAR-T) products.

Additional confirmatory testing should be performed to determine the patient’s HIV status in people who have received CAR-T treatment.

If you have any queries about a test or results interpretation please contact us.

Last updated: 11-07-2023