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ACTH (Adrenocorticotropic hormone)

Alias: Adrenocorticotropic hormone

Discipline: Clinical Chemistry

Test information: Used in investigation of Cushing's disease (abnormally high levels of cortisol) and Addison's disease (cortisol insufficiency). Please note the frontline test for Cushing's is an overnight dexamethasone suppression test or 24 hour urine cortisol, and for Addison's a 9am blood cortisol. ACTH is only indicated for the 2nd-line investigations that may follow these.

Specimen Type(s)
  • Blood;
Other Acceptable Specimen Type(s)
Specimen Container - Adult
  • Purple Top BD;
Specimen Container - Paediatric
  • Lilac Top Microtainer BD;
  • N/A;
Part of a test profile?
Volume (min) of sample to be sent to laboratory
Patient Preparation, Sample Handling and Transport
Either take sample for ACTH at 9-10am along with a paired cortisol request, or take as part of a dynamic function test.
Maximum add on time
No add ons.
Units and Reference Ranges / Interpretation
See the report.
UKAS number
UKAS accredited test?

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Last updated: 10-05-2024