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Hepatitis B Immunity

Alias: Anti-HBs, anti-Hepatitis B surface antigen antibody

Discipline: Virology

Test information: Determines the concentration of antibody to Hepatitis B surface antigen (anti-HBs) present in serum. Used to monitor the success of Hepatitis B vaccination.

Availability: Available locally Monday - Friday during routine hours: 08:45 - 17:15.

Turnaround Time: 3 Days

Specimen Type(s)
  • Clotted blood;
Other Acceptable Specimen Type(s)
Specimen Container - Adult
  • Gold Top SST BD;
Specimen Container - Paediatric
  • Plain Top Sarstedt;
  • Hepatitis B Surface antibody;
Part of a test profile?
Volume (min) of sample to be sent to laboratory
5 - 10mL
Patient Preparation, Sample Handling and Transport
  • Minimum-clotting time 30 minutes.
  • Specimens should be transported to the laboratory as soon as possible. Outside of normal hours samples should be refrigerated (2 °C to 8 °C).
  • Do NOT send grossly haemolysed specimens.
  • Please indicate vaccination history.
  • Specimens from heparinized patients may be partially coagulated, and erroneous results could occur due to the presence of fibrin.
Maximum add on time
Units and Reference Ranges / Interpretation
Units: mIU/mL
<10mIU/mL: SUSCEPTIBLE to Hepatitis B
>10 - <100mIU/mL: Protective at the time of testing, but long-term protection is uncertain.
>100mIU/mL: Post vaccination, this level of antibody confers long term protection.
UKAS number
UKAS accredited test?
  • Markers of current / past infection required in vaccine non responders.
  • Repeat vaccination course and testing 4 months post 2nd complete course.
  • Individuals with levels of <10 mIU/mL post-vaccination require Hepatitis B immunoglobulin if exposed to Hepatitis B.
  • Performance has not been established using cadaver specimens.
  • Quantitative values obtained using alternative assays may not be equivalent and cannot be used interchangeably.

If you have any queries about a test or results interpretation please contact us.

Last updated: 09-06-2023