T. whipplei PCR

Test information: A chronic multisystemic disease caused by the bacterium Tropheryma whipplei. Detection of T. whippelii by cell-culture is not a practical diagnostic test and routine serological assays are not yet available. Amplification of nucleic acid by PCR remains the preferred detection method for this organism. Please discuss this investigation with a Consultant Microbiologist first.

Availability: Referred to Great Ormond Street Hospital NHS Foundation Trust: Monday - Friday during routine hours: 08:45 - 17:15.

Turnaround Time: 7 Days upon receipt at Great Ormond Street Hospital NHS Foundation Trust

Specimen Type(s)

• Cerebrospinal fluid;
• Duodenal / Jejunal aspirates;
• Tissue Biopsies;

Other Acceptable Specimen Type(s)

N/A

Specimen Container - Adult

• Purple Top BD;
• Sterile CE-marked Universal Container;

Specimen Container - Paediatric

• N/A;

Analytes

• Tropheryma whipplei DNA;

Part of a test profile?

No

Volume (min) of sample to be sent to laboratory

EDTA Whole Blood: 2.5 - 5 mL
CSF: 200 uL

Patient Preparation, Sample Handling and Transport

• Specimens should be transported to the laboratory as soon as possible.
• Outside of normal hours samples should be refrigerated (2 °C to 8 °C).

Maximum add on time

N/A

Units and Reference Ranges / Interpretation

Results: Negative / DNA NOT Detected, Positive / DNA Detected

UKAS number

8675

UKAS accredited test?

Yes

Comments

N/A

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